應(yīng)用
Y(II)或ΔF/Fm’ 或 (Fm’ – Fs )/Fm’) 是經(jīng)受時間考驗的光適應(yīng)測量參數(shù)，比Fv/Fm對更多類型的植物脅迫更加敏感。已有的大量證據(jù)表明Fv/Fm對許多種植物脅迫和健康植物的光系統(tǒng)II的測量十分出色，而Y(II)或光量子產(chǎn)額則可測量實際光照下光適應(yīng)環(huán)境和生理狀況的光系統(tǒng)II的效率。
原理
采用調(diào)制飽和脈沖原理，測量植物的葉綠素?zé)晒?，測量參數(shù)包括植物的光量子產(chǎn)額Y(II)及相對電子傳遞速率ETR，最大光化學(xué)效率Fv/Fm，同時還可測量PAR、葉溫、相對濕度和葉片吸光率等環(huán)境參數(shù)。
特點(diǎn)
葉片吸光率測量：提供葉片吸收測量及隨環(huán)境變化導(dǎo)致的葉片吸收變化。根據(jù)Eichelman (2004) 葉片吸收在健康植物的變化范圍在0.7~0.9 之間。因此，為獲得準(zhǔn)確的ETR或“J”，Y(II)測量儀提供了一個可靠的測量方法，
Fv/Fm測量單元：用于暗適應(yīng)測量。
先進(jìn)的PAR葉夾：采用底部葉夾打開裝置，防止測量時誤操作打開葉夾。對傳感器進(jìn)行余弦校正，確保葉片相對測量光的角度不變。
Fm’校正：對于具有高光照強(qiáng)度歷史的植物，*關(guān)閉光反應(yīng)中心是一個問題，Y(II)測量儀使用Loriaux &Genty 2013的方法進(jìn)行Fm’ 校正，確保可以測得準(zhǔn)確的Fm’ 。
自動調(diào)制光設(shè)定：快速準(zhǔn)確自動的調(diào)整合適的調(diào)制光強(qiáng)，避免人工操作的誤差。
先進(jìn)算法避免飽和脈沖NPQ：采用25ms內(nèi)8點(diǎn)的平均值確定Fm、Fm’、Fo、Fs，消除飽和脈沖NPQ的影響和電子噪音。
更精確的葉溫測量：采用非接觸式紅外測量，測量精度可達(dá)±0.5℃。
直接測量相對濕度：含有測量氣體交換使用的固態(tài)傳感器，可測量相對濕度。
降低葉片遮擋的設(shè)計：傾斜的角度減少對葉片的遮擋，可以測量擬南芥等小葉。
系統(tǒng)組成
標(biāo)配： Y(II)光量子產(chǎn)額測量儀，F(xiàn)v/Fm測量儀及10個暗適應(yīng)葉夾，2個電池，2個充電器，一個便攜箱，文件U盤。
技術(shù)指標(biāo)
測量參數(shù)：
Y(II)或ΔF/Fm‘、ETR、PAR、Tleaf、相對濕度、Fms或Fm’、Fs、α(葉片吸收率)、FV/FM、FV/FO，F(xiàn)O, FM, FV。
監(jiān)測模式：允許長時間監(jiān)測
技術(shù)參數(shù)：
Y(II): 光適應(yīng)測量, 穩(wěn)態(tài)光合作用下的環(huán)境光
光源
飽和脈沖： LED白光源，使用PAR葉夾時可達(dá)7000μmols
調(diào)制光：紅光，LED 660nm，具有690nm窄通過濾器。
光化光源：環(huán)境光
檢測方法：脈沖調(diào)制法
PAR：測量400-700nm，余弦校正 ±2umols
Fv/Fm：暗適應(yīng)測量
光源：LED紅光飽和光閃，可達(dá)6000umols；
調(diào)制光：660nmLED 紅光，690nm濾波器
調(diào)制光可以根據(jù)實際測量自動調(diào)節(jié)到合適的強(qiáng)度，減少手動調(diào)節(jié)誤差，
相對濕度：0%~100%，±2%。
檢測器&過濾器：具有700~750nm帶通過濾的PIN光電二極管
可選配三腳架。
顯示：132 X 30 pixel 液晶顯示屏
取樣速率：1~10000點(diǎn)/秒自動切換。
測量時間：最短3s或也可設(shè)置長期監(jiān)測模式
存儲空間：2GB
輸出：USB下載數(shù)據(jù)，用Excel查看，無需安裝其他專用軟件
供電：USB鋰離子電池(普通充電寶)，可用8小時
尺寸：便攜箱尺寸為14”x 11”x 6”，儀器為9’’長
質(zhì)量：Y(II) 測量儀0.45 kg
Fv/Fm測量儀0.36 kg.
加便攜箱和附件總重1.95 kg.
工作溫度：0℃ ~ 50℃
產(chǎn)地
美國
文獻(xiàn)
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