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葉綠素含量測(cè)量?jī)x

簡(jiǎn)要描述:CCM-300葉綠素含量測(cè)量?jī)x是使用葉綠素?zé)晒獗嚷蕦?duì)葉綠素含量測(cè)量的一種新型儀器。與傳統(tǒng)的化學(xué)測(cè)量方法和吸收性測(cè)量方法相比,它可以對(duì)非常小的葉片以及難以測(cè)量樣品的葉綠素含量進(jìn)行非破壞性測(cè)量,測(cè)量迅速可靠。

  • 產(chǎn)品型號(hào):CCM-300
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-10-15
  • 訪  問(wèn)  量:1179

詳細(xì)介紹

    一用途

    葉綠素含量測(cè)量?jī)x廣泛應(yīng)用于植物生理生態(tài)研究,特別是對(duì)傳統(tǒng)吸收法測(cè)量困難的小葉片或樣品的葉綠素含量進(jìn)行測(cè)量,例如針葉、發(fā)育未*的水稻、生于巖石上的絲狀藻、地衣、草坪草、仙人掌、龍舌蘭屬植物、菠蘿、擬南芥、果實(shí)、苔蘚、葉莖、葉柄。

 

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    二原理

    盡管實(shí)踐已經(jīng)證明了吸收技術(shù)測(cè)量葉綠素含量的有效性,但該方法對(duì)樣品的要求較高,使其應(yīng)用范圍受到限制。而樣品本身的葉綠素?zé)晒鈩t不受樣品厚度、均一性等的影響,可以指示葉綠素的含量。根據(jù)這個(gè)原理,Gitelson等比較了葉片的吸收和反射對(duì)葉綠素?zé)晒獾挠绊?,發(fā)現(xiàn)在735nm和700nm處的比值與葉片的反射相關(guān)性最高,并且當(dāng)樣品葉綠素含量在41mg·m-2到675mg·m-2時(shí),F(xiàn)735/F700的比值與其葉綠素含量間存在著顯著的線性關(guān)系(R2≥0.95),與傳統(tǒng)的吸收性測(cè)量方法相比(測(cè)量范圍≤300mg·m-2),提高了葉綠素含量的線性關(guān)系范圍。

    根據(jù)Gitelson的研究結(jié)果,CCM-300使用具有15nm半峰寬的460nm二極管用于熒光激發(fā),提高了紅色熒光波長(zhǎng),避開(kāi)了大約685nm的發(fā)射熒光峰(葉綠素可重新吸收,降低測(cè)量精度)。儀器使用在735nm和700nm的葉綠素發(fā)射熒光的比率(F735/F700)進(jìn)行葉綠素含量的測(cè)量,讀數(shù)為F735/F700的比值以及根據(jù)Gitelson方程計(jì)算的葉綠素含量。

 

三 特點(diǎn)

 

Ø 直接算出葉綠素含量mg/m2;

 

Ø 測(cè)量結(jié)果可靠,不受葉片或樣品大小、厚度和形狀的影響;

 

Ø 經(jīng)濟(jì)、輕便、手持式設(shè)計(jì),適合野外使用;

 

Ø 與化學(xué)測(cè)量結(jié)果高度相關(guān),即使在高葉綠素含量時(shí);

 

Ø 彩色觸摸屏顯示;

 

Ø 離散的單點(diǎn)測(cè)量和多點(diǎn)(2-30個(gè)樣品)平均值測(cè)量模式;

 

Ø 非易失性閃存(2GB);

 

USB數(shù)據(jù)輸出,可使用Excel打開(kāi);

 

0001.png

 

 

 

四 組成

 

主機(jī)、光纖樣品夾、電池充電器、4節(jié)AA鎳氫充電電池、USB通訊纜線使用手冊(cè)。

 

可選配件:

 

20mm大探頭: 增加測(cè)量面積。 較大的樣本量可能對(duì)某些類型的樣本有用,例如苔蘚

 

 

0004.png

 

 

0005.png

 

 

 

增長(zhǎng)版葉夾:

 

0006.png

 

 

0007.png

 

 

 

 



技術(shù)參數(shù)

 


 

 

Ø 測(cè)量參數(shù):葉綠素?zé)晒獗嚷?/span>——發(fā)射熒光在735nm/700nm的比值(CFR),或相對(duì)葉綠素含量mg· m-2;

 

Ø 測(cè)量面積:直徑3mm的圓,外徑4mm,但對(duì)直徑小于3mm的樣品也可準(zhǔn)確可靠的測(cè)量;

 

Ø 分辨率:比值0.011 mg· m-2;

 

Ø 重復(fù)性:依賴于信號(hào)強(qiáng)度,對(duì)信號(hào)強(qiáng)度低的樣品,建議使用多點(diǎn)測(cè)量的平均值,對(duì)信號(hào)強(qiáng)度高的樣品,差異通常為±0.03或更低;。

 

Ø 噪聲:<±0.02;

 

Ø 光源:LED 460 nm藍(lán)光二極管,半峰寬15 nm;

 

Ø 探頭:兩個(gè)固態(tài)高感傳感器,提供濾光片。同時(shí)提供兩個(gè)波長(zhǎng)探頭(700nm~710nm,730nm~740nm);

 

Ø 檢測(cè):數(shù)控調(diào)制光,將背景光影響將至低。并對(duì)光源和檢測(cè)器進(jìn)行溫度補(bǔ)償;

 

Ø 存儲(chǔ)容量:2GB;

 

Ø 模式:單點(diǎn)測(cè)量,2~30個(gè)點(diǎn)去平均值,去除2σ區(qū)間外的平均值,或者中位數(shù);

 

Ø 樣品接口:光纖傳感器,直徑4mm;

 

Ø 用戶界面:240 × 320 像素彩色觸摸屏;

 

Ø 輸出:USB 1.1;

 

Ø 溫度范圍:0~50 ℃;

 

Ø 電源:2節(jié)充電AA電池;

 

Ø 自動(dòng)關(guān)閉間隔:(無(wú)按鍵或下載)0~20分鐘可選;

 

Ø 大?。?/span>12 cm × 9 cm × 3 cm

 

Ø 重量0.16lbs 275g;

 

Ø 測(cè)量時(shí)間:5秒;

 


 

 

五、產(chǎn)地:美國(guó)

 

六、參考文獻(xiàn)

 

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Casanova-Katny, (2017) Passive warming reduces stress and shifts reproductive effort in the Antarctic moss,

 

Polytrichastrum alpinum Annals of Botany, Volume 119, Issue 1, 1 January 2017, Pages 27–38,

 

 

 

 

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Volume 33, Issue 5, Part B, 25 September 2016, Pages 676-691 

 

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waterlogging during the growing season   Canadian Journal of Forest Research 2016, 46(12): 1439-1450,

 

 

 

 

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Johanna Riikonen, Nelli Kettunen, Maria Gritsevich, Teemu Hakala, Liisa Särkkä, Risto Tahvonen, (2016)

 

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Environmental and Experimental Botany Volume 121, January 2016, Pages 112-

 

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Anshu Rastogi, Marcin Stró?eckia, Hazem M. Kalaji, Dominika ?uców de Mariusz, Lamentowicz, Rados?aw

 

Juszczak (2019) Impact of warming and reduced precipitation on photosynthetic and remote sensing properties

 

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CAM植物 - 仙人掌

 

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DACTYLOPIUS OPUNTIAE (COCKERELL) (HEMIPTERA: DACTYLOPIIDAE). University of the

 

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大豆

 

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土豆

 

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Assessment of Growth, Tuber Elemental Composition, Stomatal Conductance and Chlorophyll Content of Two

 

Potato C*rs Under Irrigation with Fly Ash-Treated Acid Mine Drainage. American Journal of Potato

 

Researc August 2017, Volume 94,  Issue4, pp 367–378

 

十字花科-開(kāi)花植物

 

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Poschenrieder (2016) Characterization of Zinc and Cadmium Hyperaccumulation in Three Noccaea

 

(Brassicaceae) Populations from Non-metalliferous Sites in the Eastern Pyrenees Front Plant Sci. 2016; 7: 128.

 

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糖楓

 

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